PCR methods are the most commonly used DNA-based identity tool in the commercial food, beverage, and natural health product markets.
These methods are routinely used to identify foodborne pathogens and allergens in food. Proper validation methods for some sectors have been established, while there are none in other markets, such as botanicals. A survey of the literature indicates that some validation criteria are not addressed when developing PCR tests for botanicals.
We provide recommendations for qualitative real-time PCR methods for validating identity tests for botanical ingredients.
University of Guelph